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Micrococcal Nuclease Does Not Substantially Bias Nucleosome Mapping

We have mapped sequence-directed nucleosome positioning on genomic DNA molecules using high-throughput sequencing. Chromatins, prepared by reconstitution with either chicken or frog histones, were separately digested to mononucleosomes using either micrococcal nuclease (MNase) or caspase-activated D...

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Detalles Bibliográficos
Autores principales: Allan, James, Fraser, Ross M., Owen-Hughes, Tom, Keszenman-Pereyra, David
Formato: Artigo
Lenguaje:Inglês
Publicado: Elsevier 2012
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC3314939/
https://ncbi.nlm.nih.gov/pubmed/22310051
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmb.2012.01.043
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