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Improved Detection of Bifidobacteria with Optimised 16S rRNA-Gene Based Pyrosequencing

The 16S rRNA gene is conserved across all bacteria and as such is routinely targeted in PCR surveys of bacterial diversity. PCR primer design aims to amplify as many different 16S rRNA gene sequences from as wide a range of organisms as possible, though there are no suitable 100% conserved regions o...

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Dettagli Bibliografici
Autori principali: Sim, Kathleen, Cox, Michael J., Wopereis, Harm, Martin, Rocio, Knol, Jan, Li, Ming-Shi, Cookson, William O. C. M., Moffatt, Miriam F., Kroll, J. Simon
Natura: Artigo
Lingua:Inglês
Pubblicazione: Public Library of Science 2012
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3314643/
https://ncbi.nlm.nih.gov/pubmed/22470420
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0032543
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