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Improved Detection of Bifidobacteria with Optimised 16S rRNA-Gene Based Pyrosequencing

The 16S rRNA gene is conserved across all bacteria and as such is routinely targeted in PCR surveys of bacterial diversity. PCR primer design aims to amplify as many different 16S rRNA gene sequences from as wide a range of organisms as possible, though there are no suitable 100% conserved regions o...

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Detalhes bibliográficos
Main Authors: Sim, Kathleen, Cox, Michael J., Wopereis, Harm, Martin, Rocio, Knol, Jan, Li, Ming-Shi, Cookson, William O. C. M., Moffatt, Miriam F., Kroll, J. Simon
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3314643/
https://ncbi.nlm.nih.gov/pubmed/22470420
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0032543
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