Microtiter format gene quantification by covalent capture of competitive PCR products: application to HIV-1 detection.

We have developed a simple gene quantification system using the competitive polymerase chain reaction (CPCR) followed by microtiter format analysis. CPCR is carried out using a mutant competitor with the same size as the target DNA product, and a minimal base exchange to insure the same amplificatio...

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Bibliographic Details
Main Authors: Kohsaka, H, Taniguchi, A, Richman, D D, Carson, D A
Format: Artigo
Language:Inglês
Published: 1993
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC331446/
https://ncbi.nlm.nih.gov/pubmed/8346024
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