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Quantitation of Protein Carbonylation by Dot Blot

Protein carbonylation is the most commonly used measure of oxidative modification of proteins. It is frequently measured spectrophotometrically or immunochemically by derivatizing proteins with the classical carbonyl reagent, 2,4-dinitrophenylhydrazine. We developed an immunochemical dot blot method...

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Bibliographic Details
Main Authors: Wehr, Nancy B., Levine, Rodney L.
Format: Artigo
Language:Inglês
Published: 2012
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3314425/
https://ncbi.nlm.nih.gov/pubmed/22326366
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2012.01.031
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