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Ultrasensitive Detection of Proteins on Western Blots with Semiconducting Polymer Dots

We demonstrate ultrasensitive fluorescence imaging of proteins on Western blots using a bright, compact, and orange-emitting semiconducting polymer dot (CN-PPV). We achieved a detection limit at the single-picogram level in dot blots; with conventional Western blotting, we detected 50 pg of transfer...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Ye, Fangmao, Smith, Polina B., Wu, Changfeng, Chiu, Daniel T.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2013
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3816633/
https://ncbi.nlm.nih.gov/pubmed/23637077
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/marc.201200809
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