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Metal binding kinetics of bi-Histidine sites used in ψ-analysis: Evidence for high energy protein folding intermediates
The zinc-specific fluorophore, Zinpyr-1, is used in competition assays to determine the kinetic and thermodynamic parameters of Zn(2+) binding to engineered bi-Histidine sites located in Ubiquitin and the B domain of protein A (BdpA). These binding sites are used in ψ-analysis studies to investigate...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3313835/ https://ncbi.nlm.nih.gov/pubmed/19220047 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi802072u |
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