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Protein vivisection reveals elusive intermediates in folding

Although most folding intermediates escape detection, their characterization is crucial to the elucidation of folding mechanisms. Here we outline a powerful strategy to populate partially unfolded intermediates: A buried aliphatic residue is substituted with a charged residue (e.g., Leu→Glu(−)) to d...

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Detaylı Bibliyografya
Asıl Yazarlar: Zheng, Zhongzhou, Sosnick, Tobin R.
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: 2010
Konular:
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC2838964/
https://ncbi.nlm.nih.gov/pubmed/20144618
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmb.2010.01.056
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