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Protein vivisection reveals elusive intermediates in folding
Although most folding intermediates escape detection, their characterization is crucial to the elucidation of folding mechanisms. Here we outline a powerful strategy to populate partially unfolded intermediates: A buried aliphatic residue is substituted with a charged residue (e.g., Leu→Glu(−)) to d...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2838964/ https://ncbi.nlm.nih.gov/pubmed/20144618 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmb.2010.01.056 |
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