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Isobaric Labeling and Data Normalization without Requiring Protein Quantitation

Isobaric multiplexed quantitative proteomics can complement high-resolution sample isolation techniques. Here, we report a simple workflow exponentially modified protein abundance index (emPAI)-MW deconvolution (EMMOL) for normalizing isobaric reporter ratios within and between experiments, where sm...

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Detalhes bibliográficos
Main Authors: Kim, Phillip D., Patel, Bhavinkumar B., Yeung, Anthony T.
Formato: Artigo
Idioma:Inglês
Publicado em: Association of Biomolecular Resource Facilities 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3313697/
https://ncbi.nlm.nih.gov/pubmed/22468137
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7171/jbt.12-2301-002
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