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P214-S Identification and Quantitation of Isobarically Labeled Proteins: A Comparison of Separation Strategies
Current protocols for protein quantitation using iTRAQ reagents utilize a tryptic peptide–labeling strategy. While there are many advantages to this workflow, it precludes the effective use of protein-level separation techniques. We compare two protein-level labeling workflows. The first is in-gel d...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
The Association of Biomolecular Resource Facilities
2007
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2291909/ |
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