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Biochemical analysis of the interactions of IQGAP1 C-terminal domain with CDC42

AIM: To understand the interaction of human IQGAP1 and CDC42, especially the effects of phosphorylation and a cancer-associated mutation. METHODS: Recombinant CDC42 and a novel C-terminal fragment of IQGAP1 were expressed in, and purified from, Escherichia coli. Site directed mutagenesis was used to...

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Autores principales: Elliott, Sarah F, Allen, George, Timson, David J
Formato: Artigo
Lenguaje:Inglês
Publicado: Baishideng Publishing Group Co., Limited 2012
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC3312201/
https://ncbi.nlm.nih.gov/pubmed/22451851
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4331/wjbc.v3.i3.53
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