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Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin
Protein interactions with peptides generally have low thermodynamic and mechanical stability. Streptococcus pyogenes fibronectin-binding protein FbaB contains a domain with a spontaneous isopeptide bond between Lys and Asp. By splitting this domain and rational engineering of the fragments, we obtai...
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| Autori principali: | , , , , , , |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
National Academy of Sciences
2012
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3311370/ https://ncbi.nlm.nih.gov/pubmed/22366317 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1115485109 |
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