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An F factor based cloning system for large DNA fragments.
An effective technique using an Escherichia coli plasmid system was developed to clone fragments of exogenous DNA of as large as 100 kilobase pairs. The characteristic features of this technique are the use of a low copy number (one to two) mini-F based plasmid vector and the introduction of artific...
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| Publicado no: | Nucleic Acids Res |
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| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
1990
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| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC331087/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2197597/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/18.13.3863 |
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