Negative electron-transfer dissociation nLC-MS/MS facilitates the analysis of thousands of acidic peptides

We describe the first implementation of negative electron-transfer dissociation (NETD) on a hybrid ion trap-orbitrap mass spectrometer and its application to high-throughput sequencing of peptide anions. NETD – coupled with high pH separations, negative electrospray ionization (ESI), and an NETD com...

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Autors principals: McAlister, Graeme C., Russell, Jason D., Rumachik, Neil G., Hebert, Alexander S., Syka, John E. P., Geer, Lewis Y., Westphall, Michael S., Pagliarini, David J., Coon, Joshua J.
Format: Artigo
Idioma:Inglês
Publicat: 2012
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Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3310326/
https://ncbi.nlm.nih.gov/pubmed/22335612
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ac203430u
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