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Efficient site directed in vitro mutagenesis using ampicillin selection.
A novel plasmid vector pSELECT-1 is described which can be used for highly efficient site-directed in vitro mutagenesis. The mutagenesis method is based on the use of single-stranded DNA and two primers, one mutagenic primer and a second correction primer which corrects a defect in the ampicillin re...
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| Publié dans: | Nucleic Acids Res |
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| Auteurs principaux: | , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
Oxford University Press
1990
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| Accès en ligne: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC330994/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2194159/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/18.12.3439 |
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