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Constructing a Low-budget Laser Axotomy System to Study Axon Regeneration in C. elegans

Laser axotomy followed by time-lapse microscopy is a sensitive assay for axon regeneration phenotypes in C. elegans(1). The main difficulty of this assay is the perceived cost ($25-100K) and technical expertise required for implementing a laser ablation system(2,3). However, solid-state pulse lasers...

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Main Authors: Williams, Wes, Nix, Paola, Bastiani, Michael
格式: Artigo
語言:Inglês
出版: MyJove Corporation 2011
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC3308599/
https://ncbi.nlm.nih.gov/pubmed/22126922
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/3331
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