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Direct visualization of electrophoretic mobility shift assays using nanoparticle-aptamer conjugates
Here we demonstrate that aptamers tethered to gold nanoparticles enable direct visualization of protein-oligonucleotide interactions during gel electrophoresis. This technique is used to confirm that an aptamer previously identified as binding to C-reactive protein (CRP) only binds to the monomeric...
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| Hauptverfasser: | , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
2011
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3307786/ https://ncbi.nlm.nih.gov/pubmed/22170687 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/elps.201100308 |
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