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Elimination of nonspecific bands in non-radioactive electrophoretic mobility shift assays using the digoxigenin system
In the course of detecting nuclear transcription factors by electrophoretic mobility shift assay using digoxigenin (DIG)-labeled probes, we encountered a problem with a considerable nonspecific shift band in negative control lanes from which protein extracts were omitted. This nonspecific shift band...
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| Publicado no: | Anal Biochem |
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| Main Authors: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4318779/ https://ncbi.nlm.nih.gov/pubmed/25004462 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2014.06.020 |
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