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Cloning of the shark Po promoter using a genomic walking technique based on the polymerase chain reaction.
We have cloned the putative shark promoter of protein zero (Po) using a novel application of the ligation mediated single-sided polymerase chain reaction (PCR) method. This method uses linker ligation and subsequent amplifications with a linker primer and multiple specific primers to generate specif...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
1990
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| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC330766/ https://ncbi.nlm.nih.gov/pubmed/2339064 |
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