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Cloning of the shark Po promoter using a genomic walking technique based on the polymerase chain reaction.

We have cloned the putative shark promoter of protein zero (Po) using a novel application of the ligation mediated single-sided polymerase chain reaction (PCR) method. This method uses linker ligation and subsequent amplifications with a linker primer and multiple specific primers to generate specif...

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Détails bibliographiques
Auteurs principaux: Fors, L, Saavedra, R A, Hood, L
Format: Artigo
Langue:Inglês
Publié: 1990
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC330766/
https://ncbi.nlm.nih.gov/pubmed/2339064
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