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Cloning of the shark Po promoter using a genomic walking technique based on the polymerase chain reaction.
We have cloned the putative shark promoter of protein zero (Po) using a novel application of the ligation mediated single-sided polymerase chain reaction (PCR) method. This method uses linker ligation and subsequent amplifications with a linker primer and multiple specific primers to generate specif...
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| Publicado no: | Nucleic Acids Res |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
1990
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| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC330766/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2339064/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/18.9.2793 |
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