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DNA recombination during PCR.
PCR co-amplification of two distinct HIV1 tat gene sequences lead to the formation of recombinant DNA molecules. The frequency of such recombinants, up to 5.4% of all amplified molecules, could be decreased 2.7 fold by a 6 fold increase in Taq DNA polymerase elongation time. Crossover sites mapped e...
Tallennettuna:
| Päätekijät: | , , |
|---|---|
| Aineistotyyppi: | Artigo |
| Kieli: | Inglês |
| Julkaistu: |
1990
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| Linkit: | https://ncbi.nlm.nih.gov/pmc/articles/PMC330584/ https://ncbi.nlm.nih.gov/pubmed/2186361 |
| Tagit: |
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