DNA recombination during PCR.
PCR co-amplification of two distinct HIV1 tat gene sequences lead to the formation of recombinant DNA molecules. The frequency of such recombinants, up to 5.4% of all amplified molecules, could be decreased 2.7 fold by a 6 fold increase in Taq DNA polymerase elongation time. Crossover sites mapped e...
Shranjeno v:
| izdano v: | Nucleic Acids Res |
|---|---|
| Principais autores: | , , |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
Oxford University Press
1990
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| Online dostop: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC330584/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2186361/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/18.7.1687 |
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