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A sensitive HPLC-based method to quantify adenine nucleotides in primary astrocyte cell cultures

In mono-layered primary cell cultures baseline AMP and ADP levels are found nominally in the mid to low picomolar range and are thus difficult to measure with conventional HPLC methods that often require the pooling of samples or require indirect detection methods using radiotracers or enzyme couple...

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Detalhes bibliográficos
Main Authors: Bhatt, Dhaval P., Chen, Xuesong, Geiger, Jonathan D., Rosenberger, Thad A.
Formato: Artigo
Idioma:Inglês
Publicado em: 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3299834/
https://ncbi.nlm.nih.gov/pubmed/22382093
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jchromb.2012.02.005
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