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Creation and validation of a ligation-independent cloning (LIC) retroviral vector for stable gene transduction in mammalian cells
BACKGROUND: Cloning vectors capable of retroviral transduction have enabled stable gene overexpression in numerous mitotic cell lines. However, the relatively small number of feasible restriction enzyme sequences in their cloning sites can hinder successful generation of overexpression constructs if...
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| Главные авторы: | , , , |
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| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
BioMed Central
2012
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3298557/ https://ncbi.nlm.nih.gov/pubmed/22248071 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-12-3 |
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