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Creation and validation of a ligation-independent cloning (LIC) retroviral vector for stable gene transduction in mammalian cells

BACKGROUND: Cloning vectors capable of retroviral transduction have enabled stable gene overexpression in numerous mitotic cell lines. However, the relatively small number of feasible restriction enzyme sequences in their cloning sites can hinder successful generation of overexpression constructs if...

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Detalhes bibliográficos
Main Authors: Patel, Asmita, Muñoz, Anisleidys, Halvorsen, Katherine, Rai, Priyamvada
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3298557/
https://ncbi.nlm.nih.gov/pubmed/22248071
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-12-3
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