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Structure-Based Function Discovery of an Enzyme for the Hydrolysis of Phosphorylated Sugar Lactones

Two enzymes of unknown function from the cog1735 subset of the amidohydrolase superfamily (AHS), LMOf2365_2620 (Lmo2620) from Listeria monocytogenes str. 4b F2365 and Bh0225 from Bacillus halodurans C-125, were cloned, expressed and purified to homogeneity. The catalytic functions of these two enzym...

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Bibliographische Detailangaben
Hauptverfasser: Xiang, Dao Feng, Kolb, Peter, Fedorov, Alexander A., Xu, Chengfu, Fedorov, Elena V., Narindoshivili, Tamari, Williams, Howard J., Shoichet, Brian K., Almo, Steven C., Raushel, Frank M.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2012
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3298459/
https://ncbi.nlm.nih.gov/pubmed/22313111
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi201838b
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