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Bifunctionality of the thiamin diphosphate cofactor: assignment of tautomeric/ionization states of the 4′-aminopyrimidine ring when various intermediates occupy the active sites during the catalysis of yeast pyruvate decarboxylase

Thiamin diphosphate (ThDP) dependent enzymes perform crucial C-C bond forming and breaking reactions in sugar and amino acid metabolism and in biosynthetic pathways via a sequence of ThDP-bound covalent intermediates. A member of this superfamily, yeast pyruvate decarboxylase (YPDC) carries out the...

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Main Authors: Balakrishnan, Anand, Gao, Yuhong, Moorjani, Prerna, Nemeria, Natalia S., Tittmann, Kai, Jordan, Frank
Format: Artigo
Jezik:Inglês
Izdano: 2012
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC3295232/
https://ncbi.nlm.nih.gov/pubmed/22300533
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja211139c
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