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DNA nicking favors PCR recombination.

We attempted to use the polymerase chain reaction (PCR) to monitor in vitro recombination in a plasmid containing directly repeated sequences. Some of the plasmid preparations which had not been exposed to recombination conditions were however found to behave in the PCR test as if they had undergone...

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Detalhes bibliográficos
Main Authors: Marton, A, Delbecchi, L, Bourgaux, P
Formato: Artigo
Idioma:Inglês
Publicado em: 1991
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC329452/
https://ncbi.nlm.nih.gov/pubmed/2041780
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