Simple Cloning via Direct Transformation of PCR Product (DNA Multimer) to Escherichia coli and Bacillus subtilis

We developed a general restriction enzyme-free and ligase-free method for subcloning up to three DNA fragments into any location of a plasmid. The DNA multimer generated by prolonged overlap extension PCR was directly transformed in Escherichia coli [e.g., TOP10, DH5α, JM109, and BL21(DE3)] and Baci...

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Bibliografski detalji
Glavni autori: You, Chun, Zhang, Xiao-Zhou, Zhang, Y.-H. Percival
Format: Artigo
Jezik:Inglês
Izdano: American Society for Microbiology 2012
Teme:
Methods
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3294473/
https://ncbi.nlm.nih.gov/pubmed/22194286
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.07105-11
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