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In Vivo Characterization of Nonribosomal Peptide Synthetases (NRPSs) NocA and NocB in the Biosynthesis of Nocardicin A

Two non-ribosomal peptide synthetases (NRPS), NocA and NocB, together comprising 5 modules, are essential for the biosynthesis of the D,L,D configured tripeptide backbone of the monocyclic β-lactam nocardicin A. We report a double replacement gene strategy in which point mutations were engineered in...

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Hlavní autoři: Davidsen, Jeanne M., Townsend, Craig A.
Médium: Artigo
Jazyk:Inglês
Vydáno: 2012
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3292777/
https://ncbi.nlm.nih.gov/pubmed/22365611
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.chembiol.2011.10.020
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