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In Vivo Characterization of Nonribosomal Peptide Synthetases (NRPSs) NocA and NocB in the Biosynthesis of Nocardicin A
Two non-ribosomal peptide synthetases (NRPS), NocA and NocB, together comprising 5 modules, are essential for the biosynthesis of the D,L,D configured tripeptide backbone of the monocyclic β-lactam nocardicin A. We report a double replacement gene strategy in which point mutations were engineered in...
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| Autors principals: | , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
2012
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3292777/ https://ncbi.nlm.nih.gov/pubmed/22365611 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.chembiol.2011.10.020 |
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