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Single-molecule imaging of translational output from individual RNA granules in neurons

Dendritic RNAs are localized and translated in RNA granules. Here we use single-molecule imaging to count the number of RNA molecules in each granule and to record translation output from each granule using Venus fluorescent protein as a reporter. For RNAs encoding activity-regulated cytoskeletal-as...

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Bibliografiske detaljer
Main Authors: Tatavarty, Vedakumar, Ifrim, Marius F., Levin, Mikhail, Korza, George, Barbarese, Elisa, Yu, Ji, Carson, John H.
Format: Artigo
Sprog:Inglês
Udgivet: The American Society for Cell Biology 2012
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3290649/
https://ncbi.nlm.nih.gov/pubmed/22219377
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1091/mbc.E11-07-0622
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