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Cell-free reconstitution of vacuole membrane fragmentation reveals regulation of vacuole size and number by TORC1

Size and copy number of organelles are influenced by an equilibrium of membrane fusion and fission. We studied this equilibrium on vacuoles—the lysosomes of yeast. Vacuole fusion can readily be reconstituted and quantified in vitro, but it had not been possible to study fission of the organelle in a...

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Detalles Bibliográficos
Main Authors: Michaillat, Lydie, Baars, Tonie Luise, Mayer, Andreas
Formato: Artigo
Idioma:Inglês
Publicado: The American Society for Cell Biology 2012
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC3290646/
https://ncbi.nlm.nih.gov/pubmed/22238359
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1091/mbc.E11-08-0703
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