Quantification of nucleic acids on nitrocellulose membranes with time-resolved fluorometry.
We use a streptavidin-based macromolecular complex (SBMC) labelled with the europium chelate of 4,7-bis (chlorosulfophenyl)-1,10-phenanthroline-2,9-dicarboxylic acid (BCPDA) as a staining reagent for biotinylated DNA present on nitrocellulose filters. The fluorescent spots or bands obtained can eith...
Guardat en:
| Publicat a: | Nucleic Acids Res |
|---|---|
| Autors principals: | , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Oxford University Press
1991
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| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC329060/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1719486/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/19.21.6015 |
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