Quantification of nucleic acids on nitrocellulose membranes with time-resolved fluorometry.

We use a streptavidin-based macromolecular complex (SBMC) labelled with the europium chelate of 4,7-bis (chlorosulfophenyl)-1,10-phenanthroline-2,9-dicarboxylic acid (BCPDA) as a staining reagent for biotinylated DNA present on nitrocellulose filters. The fluorescent spots or bands obtained can eith...

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Pubblicato in:	Nucleic Acids Res
Autori principali:	Christopoulos, T K, Diamandis, E P, Wilson, G
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	Oxford University Press 1991
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC329060/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1719486/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/19.21.6015
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