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Quantification of nucleic acids on nitrocellulose membranes with time-resolved fluorometry.

We use a streptavidin-based macromolecular complex (SBMC) labelled with the europium chelate of 4,7-bis (chlorosulfophenyl)-1,10-phenanthroline-2,9-dicarboxylic acid (BCPDA) as a staining reagent for biotinylated DNA present on nitrocellulose filters. The fluorescent spots or bands obtained can eith...

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Bibliografiska uppgifter
Huvudupphovsmän: Christopoulos, T K, Diamandis, E P, Wilson, G
Materialtyp: Artigo
Språk:Inglês
Publicerad: 1991
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC329060/
https://ncbi.nlm.nih.gov/pubmed/1719486
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