Detection and Quantification of Leptospira interrogans in Hamster and Rat Kidney Samples: Immunofluorescent Imprints versus Real-time PCR

A major limitation in the clinical management and experimental research of leptospirosis is the poor performance of the available methods for the direct detection of leptospires. In this study, we compared real-time PCR (qPCR), targeting the lipL32 gene, with the immunofluorescent imprint method (IM...

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Main Authors: Chagas-Junior, Adenizar D., da Silva, Caroline L. R., Soares, Luciane Marieta, Santos, Cleiton S., M. Silva, Carlos D. C., Athanazio, Daniel A., dos Reis, Mitermayer G., Cruz McBride, Flávia W., McBride, Alan J. A.
Format: Artigo
Sprog:Inglês
Udgivet: Public Library of Science 2012
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3290571/
https://ncbi.nlm.nih.gov/pubmed/22393440
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0032712
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