Detection and Quantification of Leptospira interrogans in Hamster and Rat Kidney Samples: Immunofluorescent Imprints versus Real-time PCR

A major limitation in the clinical management and experimental research of leptospirosis is the poor performance of the available methods for the direct detection of leptospires. In this study, we compared real-time PCR (qPCR), targeting the lipL32 gene, with the immunofluorescent imprint method (IM...

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Main Authors: Chagas-Junior, Adenizar D., da Silva, Caroline L. R., Soares, Luciane Marieta, Santos, Cleiton S., M. Silva, Carlos D. C., Athanazio, Daniel A., dos Reis, Mitermayer G., Cruz McBride, Flávia W., McBride, Alan J. A.
Format: Artigo
Jezik:Inglês
Izdano: Public Library of Science 2012
Teme:
Research Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC3290571/
https://ncbi.nlm.nih.gov/pubmed/22393440
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0032712
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