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Processing in vitro of an abasic site reacted with methoxyamine: a new assay for the detection of abasic sites formed in vivo.

In this study we demonstrate that the different substrate recognition properties of bacterial and human AP endonucleases might be used to quantify and localize apurinic (AP) sites formed in DNA in vivo. By using a model oligonucleotide containing a single AP site modified with methoxyamine (MX), we...

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Udgivet i:Nucleic Acids Res
Principais autores: Rosa, S, Fortini, P, Karran, P, Bignami, M, Dogliotti, E
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 1991
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC328958/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1719478/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/19.20.5569
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