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The core element of the EcoRII methylase as defined by protease digestion and deletion analysis.
Binding of the EcoRII DNA methyltransferase to azacytosine-containing DNA protects the enzyme from digestion by proteases. The limit digest yields a product having a Mr on SDS-PAGE 20% less than the intact protein. The N terminus of the tryptic digestion product was sequenced and found to be missing...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
1991
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| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC328905/ https://ncbi.nlm.nih.gov/pubmed/1923825 |
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