The core element of the EcoRII methylase as defined by protease digestion and deletion analysis.
Binding of the EcoRII DNA methyltransferase to azacytosine-containing DNA protects the enzyme from digestion by proteases. The limit digest yields a product having a Mr on SDS-PAGE 20% less than the intact protein. The N terminus of the tryptic digestion product was sequenced and found to be missing...
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| Опубликовано в:: | Nucleic Acids Res |
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| Главные авторы: | , , |
| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
Oxford University Press
1991
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| Online-ссылка: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC328905/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1923825/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/19.19.5403 |
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