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Oligodeoxyribonucleotide ligation to single-stranded cDNAs: a new tool for cloning 5' ends of mRNAs and for constructing cDNA libraries by in vitro amplification.

Cloning full length cDNAs is a difficult task especially if mRNAs are not abundant or if tissue is only available in limited amounts. Current strategies are based on in vitro amplification of cDNAs after adding a homopolymeric tail at the 3' end of the ss-cDNA. Since subsequent amplification st...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Prif Awduron: Edwards, J B, Delort, J, Mallet, J
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: 1991
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC328880/
https://ncbi.nlm.nih.gov/pubmed/1923806
Tagiau: Ychwanegu Tag
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