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Monoclonal antibody-based, selective isolation of DNA fragments containing an alkylated base to be quantified in defined gene sequences.

We have established a sensitive, monoclonal antibody (Mab)-based procedure permitting the selective enrichment of sequences containing the miscoding alkylation product O6-ethylguanine (O6-EtGua) from mammalian DNA. H5 rat hepatoma cells were reacted with the N-nitroso carcinogen N-ethyl-N-nitrosoure...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Hochleitner, K, Thomale, J, Nikitin AYu, Rajewsky, M F
Format: Artigo
Sprache:Inglês
Veröffentlicht: 1991
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC328635/
https://ncbi.nlm.nih.gov/pubmed/1886770
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