Purification of BsuE methyltransferase and its application in genome mapping.
We have used a combination of BsuE methyltransferase (M-BsuE) and NotI restriction enzyme to cut genomic DNA at a subset of NotI sites. The usefulness of this system is shown in a re-examination of the restriction map of the human MHC. Combinations of methylases and restriction enzymes can be used t...
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| Veröffentlicht in: | Nucleic Acids Res |
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| Hauptverfasser: | , , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Oxford University Press
1991
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| Online-Zugang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC328567/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1870977/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/19.15.4233 |
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