Purification of BsuE methyltransferase and its application in genome mapping.

We have used a combination of BsuE methyltransferase (M-BsuE) and NotI restriction enzyme to cut genomic DNA at a subset of NotI sites. The usefulness of this system is shown in a re-examination of the restriction map of the human MHC. Combinations of methylases and restriction enzymes can be used t...

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Detalhes bibliográficos

Publicado no:	Nucleic Acids Res
Principais autores:	Shukla, H, Kobayashi, Y, Arenstorf, H, Yasukochi, Y, Weissman, S M
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Oxford University Press 1991
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC328567/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1870977/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/19.15.4233
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