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Isolation of estrogen receptor-binding sites in human genomic DNA.
Total genomic DNA digested by restriction enzymes was mixed with the DNA-binding domain of the estrogen receptor (ER-DBD) that was expressed in Escherichia coli and the fragments that bound to it were selected by nitrocellulose filter. These fragments were cloned into a plasmid vector and amplified....
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
1991
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC328545/ https://ncbi.nlm.nih.gov/pubmed/1870965 |
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