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A hammerhead ribozyme allows synthesis of a new form of the Tetrahymena ribozyme homogeneous in length with a 3' end blocked for transesterification.

The L-21 Scal form of the Tetrahymena ribozyme acts as a sequence-specific endonuclease. This ribozyme has a homogeneous 5' end but a somewhat heterogeneous 3' end, as is typical of RNA synthesized by transcription in vitro. To produce a more homogeneous ribozyme for both structural and enzymologica...

תיאור מלא

שמור ב:
מידע ביבליוגרפי
הוצא לאור ב:Nucleic Acids Res
Principais autores: Grosshans, C A, Cech, T R
פורמט: Artigo
שפה:Inglês
יצא לאור: Oxford University Press 1991
גישה מקוונת:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC328477/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1650453/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/19.14.3875
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