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A hammerhead ribozyme allows synthesis of a new form of the Tetrahymena ribozyme homogeneous in length with a 3' end blocked for transesterification.

The L-21 Scal form of the Tetrahymena ribozyme acts as a sequence-specific endonuclease. This ribozyme has a homogeneous 5' end but a somewhat heterogeneous 3' end, as is typical of RNA synthesized by transcription in vitro. To produce a more homogeneous ribozyme for both structural and en...

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Dettagli Bibliografici
Autori principali: Grosshans, C A, Cech, T R
Natura: Artigo
Lingua:Inglês
Pubblicazione: 1991
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC328477/
https://ncbi.nlm.nih.gov/pubmed/1650453
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