Imaging the early stages of phospholipase C/sphingomyelinase activity on vesicles containing coexisting ordered-disordered and gel-fluid domains

The binding and early stages of activity of a phospholipase C/sphingomyelinase from Pseudomonas aeruginosa on giant unilamellar vesicles (GUV) have been monitored using fluorescence confocal microscopy. Both the lipids and the enzyme were labeled with specific fluorescent markers. GUV consisted of a...

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Main Authors: Ibarguren, Maitane, López, David J., Montes, L.-Ruth, Sot, Jesús, Vasil, Adriana I., Vasil, Michael L., Goñi, Félix M., Alonso, Alicia
Formáid: Artigo
Teanga:Inglês
Foilsithe: The American Society for Biochemistry and Molecular Biology 2011
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Research Articles
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC3284157/
https://ncbi.nlm.nih.gov/pubmed/21252263
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1194/jlr.M012591
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