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Purification and characterisation of the TnsB protein of Tn7: a transposition protein that binds to the ends of Tn7.
Tn7, a large bacterial transposon encodes 5 proteins required for its transposition. We report a rapid and easy purification of one of these proteins, TnsB, from an overexpression strain. This protein was shown to bind to the ends of Tn7, in a bandshift assay, in two distinct stages as a function of...
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| Veröffentlicht in: | Nucleic Acids Res |
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| Hauptverfasser: | , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Oxford University Press
1991
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| Online Zugang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC328340/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1648205/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/19.12.3395 |
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