Mapping irradiation hybrids to cosmid and yeast artificial chromosome libraries by direct hybridization of Alu-PCR products.

A direct hybridization protocol is described for screening cosmid and yeast artificial chromosome libraries with pools of Alu-PCR products from somatic cell or irradiation hybrids. This method eliminates purification, cloning and analysis of each individual Alu-PCR product before library screening....

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Bibliografiske detaljer
Main Authors: Monaco, A P, Lam, V M, Zehetner, G, Lennon, G G, Douglas, C, Nizetic, D, Goodfellow, P N, Lehrach, H
Format: Artigo
Sprog:Inglês
Udgivet: 1991
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC328328/
https://ncbi.nlm.nih.gov/pubmed/2062647
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