Mapping irradiation hybrids to cosmid and yeast artificial chromosome libraries by direct hybridization of Alu-PCR products.

A direct hybridization protocol is described for screening cosmid and yeast artificial chromosome libraries with pools of Alu-PCR products from somatic cell or irradiation hybrids. This method eliminates purification, cloning and analysis of each individual Alu-PCR product before library screening....

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Pubblicato in:	Nucleic Acids Res
Autori principali:	Monaco, A P, Lam, V M, Zehetner, G, Lennon, G G, Douglas, C, Nizetic, D, Goodfellow, P N, Lehrach, H
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	Oxford University Press 1991
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC328328/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2062647/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/19.12.3315
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