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A Thioester Substrate Binds to the Enzyme Arthrobacter Thioesterase in Two Ionization States; Evidence from Raman Difference Spectroscopy

4-Hydroxybenzoyl-CoA (4-HB-CoA) thioesterase from Arthrobacter is the final enzyme catalyzing the hydrolysis of 4-HB-CoA to produce coenzyme A and 4-hydroxybenzoic acid in the bacterial 4-chlorobenzoate dehalogenation pathway. Using a mutation E73A that blocks catalysis, stable complexes of the enzy...

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Autors principals: Dong, Jian, Zhuang, Zhihao, Song, Feng, Dunaway-Mariano, Debra, Carey, Paul R.
Format: Artigo
Idioma:Inglês
Publicat: 2012
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3280504/
https://ncbi.nlm.nih.gov/pubmed/22347769
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/jrs.3002
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